Case Of The Crown Jewels Template Page 21
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1
ESTRICTION
NZYME
ORKSHEET
N
:
AME
A natural enemy of bacteria is a virus. To defend them when attacked by a virus, bacteria use chemical
weapons that breakup the DNA of the virus. The action of these chemicals on the viral DNA is shown in
the diagram below:
cut
cut
DNA from Virus
TACCGGG AATTCATCCGGTG AATTCTAGCGTAC
ATGGCCCTTAA GTAGGCCACTTAA GATCGCATG
TACCGGG
AATTCATCCGGTG
AATTCTAGCGTAC
ATGGCCCTTAA
GTGGCCACTTAA
GATCGCATG
Use the diagram above to complete the sentences or answer the questions below:
1.
The chemical that cuts the DNA is called a restriction enzyme. Restriction enzymes cut the
DNA into
.
2.
The restriction enzyme used above is called EcoRI. EcoRI cuts DNA everywhere the base
pattern
is found.
3.
Another restriction enzyme is HaeIII. It cuts DNA at the base sequence CCGG. It cuts
between the C and G. Show the DNA fragments that would result if HaeIII was used to cut the
DNA fragment shown in the diagram above.
4.
Do you think restriction enzymes could be used to cut DNA from other organisms?
R e s t r i c t i o n E n z y m e W o r k s h e e t 1
P a g e 19
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