CONFIDENTIAL
C
P
D
ITY
OLICE
EPARTMENT :
D
/O
S
ATA
BSERVATION
HEET
I. R
D
ESTRICTION
IGEST
1.
What does the restriction enzyme do to the DNA?
2.
Why are the DNA samples put into the incubator?
3.
Predict what will happen to the DNA you prepared in part I while it is in the incubator.
II. P
A
G
REPARATION OF THE
GAROSE
EL
1.
What is the function of the Agarose gel?
2.
Predict what would happen if you used 0.02 g of Agarose instead of 0.2 g. What effect would
that have on the experiment?
3.
What is the function of the comb?
D a t a / O b s e r v a t i o n S h e e t
P a g e 17