Water Microbiology Laboratory Evaluation Form - Illinois Department Of Public Health Page 7
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22WATER MICROBIOLOGY LABORATORY EVALUATION FORM
State of Illinois
Illinois Department of Public Health
LABORATORY EQUIPMENT, SUPPLIES AND MATERIALS (cont’d)
19. Dilution Bottles
a. Dilution bottles of borosilicate glass or other corrosion resistant glass or of autoclavable plastic
(465.340g).
______
b. Graduation level distinctly marked at 99 ml (465.340g).
______
QC c. Plastic screw caps with leak proof liner free of toxicity by test (465.340g).
______
20. Sample Containers
a. Capacity at least 120 ml (4 oz) to allow at least one inch head space (465.340h).
______
b. Sample bottles wide mouth plastic with a non-toxic cap liner, borosilicate glass with a ground glass stopper,
pre-sterilized containers, including single-service sterilized plastic bottles, or sampling bags with sodium
thiosulfate (465.340h).
______
c. Tops of glass-stoppered bottles covered with aluminum foil or char-resistant paper prior to sterilization
(465.340h).
______
d. Glass bottles sterilized by autoclaving or dry heat; plastic bottles sterilized by autoclaving (465.350a1,
465.350a5).
______
e. Empty container moistened before autoclaving (465.350a6).
______
21. Miscellaneous Supplies
a. Glass made of borosilicate or other corrosion-resistant glass (465.340a).
______
b. Free of chips, cracks or excessive etching (465.340a).
______
c. Plastic items are non-toxic (465.340a).
______
d. Graduated cylinders and other pre-calibrated containers used to measure sample volume have clearly
marked volumes of 2.5% tolerance or less (465.340b).
______
e. Media preparation utensils borosilicate glass or stainless steel, clean and dry, free from foreign residues
or dried medium (465.340c).
______
22. Ultraviolet Lamp for Funnel Disinfection
______
QC a. Lamps cleaned monthly with a soft cloth moistened with ethanol (465.400w).
______
QC b. Lamp tested quarterly by exposing agar spread plates to the light for two minutes; alternatively lamps
checked upon first use and quarterly with light meter (465.400w).
______
QC c. Lamps replaced if less than 99% kill or if emission < 70% of initial output (465.400w).
______
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