Water Microbiology Laboratory Evaluation Form - Illinois Department Of Public Health Page 6
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22WATER MICROBIOLOGY LABORATORY EVALUATION FORM
State of Illinois
Illinois Department of Public Health
LABORATORY EQUIPMENT, SUPPLIES AND MATERIALS (cont’d)
14. Membrane Filtration (MF) Equipment
Manufacturer/Model __________________________________________________________________
a. MF units of stainless steel, glass or autoclavable plastic (465.330o).
______
b. Units do not leak, not scratched or corroded (465.330o).
______
c. Forceps tips without corrugations (465.330r).
______
QC d. Multi-use MF units initially calibrated with Class A graduated cylinder; tolerance ± 2.5% (465.400ff).
______
QC e. Each lot of single use MF units checked for calibration with Class A graduated cylinder; ± 2.5% tolerance
(465.400ee).
______
15. Membrane Filters and Pads
Manufacturer/Model __________________________________________________________________
a. Membrane filters from cellulose ester material, white, grid marked, 47mm diameter, 0.45μm pore size
______
(465.330p).
b. Alternate pore size used if manufacturer gives performance data equal to or better than the 0.45μm
membrane filter (465.330p).
______
c. Membrane filters recommended by manufacturer for water analysis (465.400g).
______
d. Membrane filters and pads purchased presterilized or autoclaved before use (465.330p) (465.330q).
______
QC e. One certificate per lot number of membrane filters on file; date of receipt recorded (465.400g).
______
f. Membrane filters not brittle or distorted, no gridline inhibition (465.400g).
______
QC g. Run positive control on each new lot (465.400g).
______
16. Culture Dishes
a. Pre-sterilized plastic or sterilized glass dishes used (465.340e).
______
b. Loose-lid dishes incubated in a tight-fitting container (465.340e).
______
c. Glass culture dishes sterilized in stainless steel or aluminum canisters or in heavy aluminum foil or
char-resistant paper (465.340e).
______
d. Open packs of disposable culture dishes resealed between uses (465.340e).
______
e. Dishes clear, flat bottomed and free from bubbles and scratches (465.340e).
______
17. Culture Tubes, Containers, and Closures
a. Tubes and containers borosilicate glass or other corrosion-resistant glass (465.340f).
______
b. Tubes and containers of sufficient size that medium plus sample does not exceed 3/4 full (465.340f).
______
c. Closures stainless steel, plastic, aluminum or screw cap with non-toxic liner (465.340f).
______
d. Cotton and foam plugs not allowed (465.340f).
______
18. Pipettes
a. Reusable pipettes sterilized in stainless steel or aluminum canisters (465.340d).
______
b. Packs of disposable sterile pipettes resealed between major use periods (465.340d).
______
c. Pipettes not etched or chipped, graduation markings legible (465.340a).
______
d. Pipettes and pipettors have a tolerance of 2.5% or less (465.340d) (465.400u).
______
e. Micropipettes are fixed volume, tips sterile, calibrated annually with 10 weighings, or for volumes greater
than or equal to 1 ml checked with a Class A graduated cylinder (465.400u).
______
f. Pipetting devices used; mouth pipetting not permitted (465.340d).
______
g. Pipette aid clean and dry; no pipette aids allowed that were previously used outside of the certified
laboratory (465.340d).
______
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