Water Microbiology Laboratory Evaluation Form - Illinois Department Of Public Health Page 4
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22WATER MICROBIOLOGY LABORATORY EVALUATION FORM
State of Illinois
Illinois Department of Public Health
LABORATORY EQUIPMENT, SUPPLIES AND MATERIALS (cont’d)
4.
pH Meter
Manufacturer/Model __________________________________________________________________
a. Accuracy of ± 0.1 units; scale graduation ± 0.1 units (465.330c).
______
b. Electrodes maintained according to manufacturer's recommendations (465.400a).
______
c. pH buffer solution aliquots used only once (465.400a).
______
QC d. Commercial buffer solutions dated when received and discarded before expiration date (465.400a).
______
QC e. pH meter standardized each day of use with pH 7.0 and either pH 4.0 or pH 10.0 standard buffers;
record of the standardization including percent slope maintained (percent slope 95%-105%)(465.400a).
______
5.
Agar Tempering Water Bath
Manufacturer/Model __________________________________________________________________
______
a. Appropriate size for holding melted media (465.330j).
______
QC b. Temperature maintained at 45 ± 1°C ( 465.330j).
______
6.
Incubator Unit(s)
Manufacturer/Model (35°)______________________________________________________________
Manufacturer/Model (44.5°) ____________________________________________________________
Manufacturer/Model (spore) ____________________________________________________________
a. Maintains uniform temperature of 35°C ± 0.5°C, 44.5°C ± 0.2°C, (465.330g); water bath circulating with
cover (465.330g).
______
QC b. Temperatures recorded continuously or recorded twice daily (at times separated by at least four hours)
(465.400d).
______
c. Thermometers on top and bottom shelves of the use area (465.400d).
______
d. Temperature readings from walk-in incubators with a continuous reading device supplemented by
readings from thermometers placed on shelves other than where the device is located (465.400d).
______
e. Thermometer bulb immersed in liquid (465.400d).
______
f. Culture dishes and tubes in aluminum block incubator fit snugly (465.330g).
______
7.
Refrigerator
Manufacturer/Model __________________________________________________________________
a. Temperature maintained at 1°C to 5°C on top shelf (465.330i).
______
b. Thermometer graduated in 1°C increments or less (465.330i).
______
c. Thermometer bulb immersed in liquid (465.330i) (465.400v).
______
QC d. Temperature recorded daily (465.400v).
______
e. Refrigerator unit visibly clean and outdated materials discarded (465.400v).
______
8.
Autoclave
Manufacturer/Model __________________________________________________________________
a. Separate pressure and temperature gauges with sensor on exhaust (465.330e3).
______
b. Operational safety valve (465.330e2).
______
c. Sterilization temperature (121 ± 1°C) maintained during cycle (465.330e4).
______
d. Entire cycle completed within 45 minutes when a 12 minute-15 minute sterilization period used
(465.330e4).
______
4
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