Water Microbiology Laboratory Evaluation Form - Illinois Department Of Public Health Page 19
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22WATER MICROBIOLOGY LABORATORY EVALUATION FORM
State of Illinois
Illinois Department of Public Health
METHODOLOGY (cont’d)
QC o. Media control for each bottle of agar used (poured last); air control exposed for 15 minutes, pipet,
petri dishes, and dilution water controls for each series of samples (air plate started before making
first dilution or plating first sample) (465.390d1,d2,d3,d4).
______
QC p. Agar weight loss determined quarterly, less than 15%.
______
q. Counts reported for plates having 30 colonies -300 colonies (If 1.0 ml of undiluted sample results in
fewer than 30 colonies, actual count reported) (465.360t9).
______
r.
If more than one analyst in laboratory, each counted the colonies on same plate monthly;
colony counts agreed within 10% (465.390c4)(SWTR).
______
s. SimPlate Unit Dose: 10 mL added to dehydrated medium or 9 mL sterile diluent and 1 mL sample,
poured into center of plate, distributed evenly, excess drained into absorbent pad (465.360t7).
______
t.
SimPlates incubated in inverted position at 35°C ± 0.5°C for 45 hours - 72 hours (465.360t7).
______
u. Wells that fluoresce under UV light counted, count converted with Idexx Unit Dose MPN table
(465.360t7).
______
v.
10 mL sample most probable number (MPN) read directly, 1 mL sample multiplied by 10 (465.360t7).
______
w. SimPlate Multiple Dose: 100 mL sterile diluent added to dehydrated medium, 1.0 mL of sample and
9 mL of reconstituted medium added to center of plate, plate swirled to mix, distributed evenly,
excess drained into absorbent pad (465.360t8).
______
x. SimPlates incubated in inverted position at 35°C ± 0.5°C for 45 hours - 72 hours (465.360t7).
______
y.
Wells that fluoresce counted under UV light; count converted with Idexx Multi-Dose MPN table;
if dilution made, MPN value multiplied by dilution factor (465.360t7).
______
19
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