Water Microbiology Laboratory Evaluation Form - Illinois Department Of Public Health Page 17
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22WATER MICROBIOLOGY LABORATORY EVALUATION FORM
State of Illinois
Illinois Department of Public Health
METHODOLOGY (cont’d)
1. Positive culture from m-Endo, m-Endo LES or m-FC transferred to EC-MUG.
______
2. Water level of water bath above upper level of media in culture tubes.
______
3. Incubated at 44.5°C ± 0.2°C for 24 ± 2 hours.
______
4. Fluorescence considered E. coli positive.
______
c. Escherichia coli NA-MUG Procedure following membrane filtration methods
RTCR (Detect)
LT2 (Count)
SM 20th Ed 9222G.1c(1)
E. coli
SM 20th Ed 9222G.1c(1)
E. coli
SM 21st Ed 9222G.1c(1)
1. Membrane filter transferred from 22 hours -24 hours m-Endo plate or 22 hours - 26 hours m-FC
plate to NA-MUG.
______
2. Sheen (m-Endo) or blue (m-FC) colonies marked on lid; incubated additional four hours at
35°C ± 0.5°C.
______
3. Fluorescent colonies reported as E. coli.
______
7. Enzyme Substrate Methods
a. Colilert, Colilert 18, Colisure
RTCR (Detect)
SWTR (Count)
SM 20th Ed 9223B
SM 20th Ed 9223B
Total Coliform
Total Coliform
SM 21st Ed 9223B
SM 21st Ed 9223B
and E. coli
and E. coli
SM online 9223B-97
SM online 9223B-97
b.
Readycult®, E*Colite®, Modified Colitag™
RTCR (Detect)
Readycult® Manufacturer’s Instructions
Total Coliform
E*Colite® Manufacturer’s Instructions
and E. coli
Modified Colitag™ Manufacturer’s Instructions
c. Colilert/Colilert 18 with Quantitray or Quantitray 2000, or MTF (5-10 tubes) used for SWTR samples;
dilution water, if used, is sterile deionized water or distilled water (465.360k14).
______
d. Colilert Test: samples incubated at 35°C ± 0.5°C for 24 hours, up to 28 hours; yellow,
coliform positive (465.360k13).
______
e. Colilert 18 Test: samples incubated at 35°C ± 0.5°C for 18 hours, up to 22 hours; yellow,
coliform positive (465.360k13).
______
f.
Colisure Test: samples incubated at 35°C ± 0.5°C for 24 hours, up to 48 hours; red/magenta,
coliform positive (465.360k16).
______
g. E*Colite Test: samples incubated at 35°C ± 0.5°C for 28 hours; blue/blue-green, coliform positive; add
20 hours incubation if no fluorescence; if red, sample discarded and resample requested (465.360k17).
______
h. Readycult Test: samples incubated at 35°C ± 0.5°C for 24 ±1 hours; blue-green, coliform positive
______
(465.360k18).
i.
Modified Colitag Test: samples incubated at 35°C ± 0.5°C for 24 ±2 hours; yellow, coliform positive
(465.360k19).
______
17
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