Water Microbiology Laboratory Evaluation Form - Illinois Department Of Public Health Page 16
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22WATER MICROBIOLOGY LABORATORY EVALUATION FORM
State of Illinois
Illinois Department of Public Health
METHODOLOGY (cont’d)
5. Fecal Coliform Fermentation Broth Methods
SWTR (Count)
SWTR (Count)
SM 20th Ed 9221E
SM 20th Ed 9221E
Fecal Coliform
SM 21st Ed 9221E
SM 21st Ed 9221E
Fecal Coliform
(EC Broth)
(A-1 Broth)
SM 22nd Ed 9221E
SM 22nd Ed 9221E
SM online 9221E-99
a. A-1 Broth (EPA Manual)
1. Three sample volumes of source water (e.g., 10, 1, 0.1 mL), or five or 10 tubes/sample volume used. ______
NA
2. Incubated three hours at 35°C ± 0.5°C.
______
NA
3. Tubes transferred to 44.5°C ± 0.2°C water bath and incubated additional 21 ± 2 hours.
______
NA
4. Any gas detected in inverted vial of tube that has turbid growth reported as positive for fecal coliform. ______
NA
5. Water level of water bath above level of media in culture tubes.
______
NA
b. EC Broth (EPA Manual)
1. Initially, MTF test conducted, (presumptive phase).
______
NA
2. Three sample volumes of source water (e.g., 10, 1, 0.1 mL), or five or 10 tubes/sample volume used. ______
NA
3. Incubated at 44.5°C ± 0.2°C for 24 ± 2 hours.
______
NA
4. Any gas detected in inverted vial of tube that has turbid growth reported as positive for fecal coliform. ______
NA
5. Water level of water bath above level of media in culture tubes.
______
NA
6. Verification Procedures
a. Escherichia coli Procedure following lactose fermentation methods
RTCR (Detect)
GWR (Detect)
SM 20th Ed 9221F.1
E. coli
SM 22nd Ed 9221F
E. coli
SM 21st Ed 9221F.1
1. Positive culture from LTB transferred to EC-MUG.
______
2. Water level of water bath above upper level of media in culture tubes.
______
3. Incubated at 44.5°C ± 0.2°C for 24 ± 2 hours.
______
4. Fluorescence considered E.coli positive.
______
b. Escherichia coli Partition Method following Membrane Filtration Methods
RTCR (Detect)
LT2 (Count)
SM 20th Ed 9222G.1c(2)
E. coli
SM 20th Ed 9222G
E. coli
SM 21st Ed 9222G.1c(2)
GWR (Detect)
E. coli
SM 20th Ed 9222G
16
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