Water Microbiology Laboratory Evaluation Form - Illinois Department Of Public Health Page 11
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22WATER MICROBIOLOGY LABORATORY EVALUATION FORM
State of Illinois
Illinois Department of Public Health
ANALYTICAL MEDIA
1. General
a. Either commercially prepared or commercially dehydrated media used (465.350d1).
______
QC b. Records kept of kind, amount, date received and date opened for containers of media (465.400y).
______
c. Media used on first in first out basis (465.400y).
______
d. Date received and date opened (initial use) written on containers (465.400y).
______
e. Dehydrated media stored in cool, dry location (465.350d3).
______
f.
Opened bottles of dehydrated media kept in desiccator.
Yes _____ No _____
______
g. Media that have passed manufacturer's expiration date discarded (465.400y).
______
h. Open dehydrated media discarded after six months (by manufacturer’s expiration date if stored in
desiccator) (465.400y).
______
i.
Media discarded if visible deterioration is observed; e.g. clumping, color change, visible growth,
sheen (465.400y) (465.360m6).
______
j.
Prepared agar refrigerated, placed in tightly closed container, dish or plastic bag; laboratory prepared
MF agar used within two weeks; laboratory prepared MF broth used within 96 hours (465.360m6).
______
k.
Multiple Tube Fermentation (MTF) broth with loose-fitting caps used within one week (465.350d6).
______
QC l.
Lab prepared MTF broth with screw caps used within three months stored in the dark;
evaporation <1.0 mL per 10 mL (465.350d7).
______
m. Refrigerated sterilized MTF broth incubated overnight at 35°C; tubes with growth or gas bubbles
discarded (465.350d6).
______
QC n. Media preparation records include type of medium, lot number, date of preparation, sterilization time
and temperature, final pH, initials (465.400n).
______
QC o. Media dispensing apparatus, when used, checked for accuracy (465.400t).
______
QC p. Each new lot/batch of medium checked with known positive and negative culture controls before use
(465.400p).
______
q. If lactose broth used, 25 parallel tests with lauryl tryptose broth (LTB) conducted before first use;
results differ <10% (465.400r).
______
r.
M-Endo broth, M-Endo agar LES and m-FC media prepared in sterile flask; brought just to boiling point,
not autoclaved (465.360m1) (465.360m5).
______
s. Rosolic acid (1% in 0.2N NaOH; not autoclaved) added to m-FC media when heavy background
anticipated (SM9222D) (SM9222D-97).
______
t.
MI agar melted in boiling water bath or according to manufacturer’s recommendation, not autoclaved
(EPA Manual).
______
u. Filter sterilized cefsulodin added to tempered MI agar (EPA Manual).
______
QC v.
Commercially prepared media records include: date received, type of medium, lot number, sample
performance and pH (465.400o).
______
11
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